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1

Microscale isolation of native forms of lysozyme from chicken egg white by gel isoelectric focusing

Shimazaki, Youji, Ochi, Yoshiko, Fujimura, Kennosuke

Journal:

ELECTROPHORESIS

Year:

2018

Language:

english

File:

PDF, 900 KB

english, 2018

2

Analysis of lipid hydrolytic activity by esterase on blotting membrane followed by separation using non-denaturing two-dimensional gel electrophoresis

Youji Shimazaki, Masayuki Miyamoto

Journal:

Biotechnology and Bioengineering

Year:

2007

Language:

english

File:

PDF, 190 KB

english, 2007

3

Simultaneous analysis of esterase and transferase activities in cytosol proteins from the bovine retina by using microscale non-denaturing two-dimensional electrophoresis

Youji Shimazaki, Yoko Hiraka, Minatsu Uesugi, Takashi Manabe

Journal:

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

Year:

2004

Language:

english

File:

PDF, 426 KB

english, 2004

4

Activity and sequence structure analysis of cytosolic dehydrogenase by mass spectrometry after separation by nondenaturing two-dimensional electrophoresis

Youji Shimazaki, Yuki Sugawara

Journal:

Analytical Biochemistry

Year:

2004

Language:

english

File:

PDF, 257 KB

english, 2004

5

Production of immunoaffinity membranes for direct analysis of antigen after antibody separation and blotting under non-denaturing conditions

Youji Shimazaki, Azusa Kodama

Journal:

Analytica Chimica Acta

Year:

2009

Language:

english

File:

PDF, 806 KB

english, 2009

6

Detection of activity and mass spectrometric identification of mouse liver carboxylesterase and aldehyde dehydrogenase separated by non-denaturing two-dimensional electrophoresis after extraction with detergents

Youji Shimazaki, Takashi Manabe

Journal:

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

Year:

2005

Language:

english

File:

PDF, 808 KB

english, 2005

7

Analysis of hydrolytic activity of phospholipase Cα from porcine retina on retinyl ester and phosphatidylcholine using non-denaturing two-dimensional electrophoresis and mass spectrometry

Youji Shimazaki, Haruka Kishi, Masami Mori, Chiyo Yasuda, Takashi Manabe

Journal:

Journal of Chromatography B

Year:

2006

Language:

english

File:

PDF, 432 KB

english, 2006

8

Simultaneous production of immunoaffinity membranes

Youji Shimazaki, Masayuki Miyamoto

Journal:

Journal of Chromatography B

Year:

2010

Language:

english

File:

PDF, 437 KB

english, 2010

9

Analysis of the activity and identification of enzymes after separation of cytosol proteins in mouse liver by microscale nondenaturing two-dimensional electrophoresis

Youji Shimazaki, Yuki Sugawara, Yuki Ohtsuka, Takashi Manabe

Journal:

Year:

2003

Language:

english

File:

PDF, 180 KB

english, 2003

10

Nondenaturing two-dimensional electrophoresis enzyme profile involving activity and sequence structure of cytosol proteins from mouse liver

Youji Shimazaki, Yuki Sugawara, Takashi Manabe

Journal:

Year:

2004

Language:

english

File:

PDF, 129 KB

english, 2004

11

Localization of a visual Gq protein in the photoreceptors of a polychaete,Perinereis brevicirris(Annelida)

Y. Miyako-Shimazaki, Youji Shimazaki, Kohzoh Ohtsu, Masamichi Yamamoto

Journal:

Cell and Tissue Research

Year:

1999

Language:

english

File:

PDF, 422 KB

english, 1999

12

Production of enzyme reactors after separation by non-denaturing two-dimensional electrophoresis and immobilization on membrane

Youji Shimazaki, Takahiro Kuroda

Journal:

Biotechnology Letters

Year:

2009

Language:

english

File:

PDF, 184 KB

english, 2009

13

Reversible Inhibition of Esterase Activity After Separation and Immobilization

Takahiro Sakikawa, Youji Shimazaki

Journal:

Applied Biochemistry and Biotechnology

Year:

2011

Language:

english

File:

PDF, 179 KB

english, 2011

14

Simultaneous immobilization of dehydrogenases on polyvinylidene difluoride resin after separation by non-denaturing two-dimensional electrophoresis

Youji Shimazaki, Mariko Kadota

Journal:

Analytica Chimica Acta

Year:

2008

Language:

english

File:

PDF, 865 KB

english, 2008

15

Direct analysis of retinal dehydrogenase activity on an electroblotting membrane following separation by non-denaturing two-dimensional electrophoresis

Youji Shimazaki, Takahiro Kuroda

Journal:

Journal of Chromatography B

Year:

2007

Language:

english

File:

PDF, 465 KB

english, 2007

16

Successive analysis of antigen trapping and enzymatic digestion on membrane-immobilized avidin

Youji Shimazaki, Yoshinori Kohno

Journal:

Analytical Biochemistry

Year:

2012

Language:

english

File:

PDF, 498 KB

english, 2012

17

Hydrolytic activity of lipase on anion-exchange solid phase column after separation and electrotransfer by non-denaturing electrophoresis

Youji Shimazaki

Journal:

Journal of Biochemical and Biophysical Methods

Year:

2007

Language:

english

File:

PDF, 525 KB

english, 2007

18

Direct trapping and analysis of hemoglobin in flowing fluid using membrane-immobilized anti-haptoglobin antibody

Youji Shimazaki, Ayaka Kimura

Journal:

Journal of Pharmaceutical and Biomedical Analysis

Year:

2011

Language:

english

File:

PDF, 295 KB

english, 2011

19

Silver staining of an esterase compatible with activity and mass spectrometry analysis after separation using non-denaturing two-dimensional electrophoresis

Youji Shimazaki, Sono Watanabe

Journal:

Clinica Chimica Acta

Year:

2008

Language:

english

File:

PDF, 324 KB

english, 2008

20

Maintenance of esterase activity and immunoreactivity after colloidal silver staining of non-denaturing proteins

Youji Shimazaki, Seigo Horikawa

Journal:

Clinica Chimica Acta

Year:

2010

Language:

english

File:

PDF, 121 KB

english, 2010

21

Analysis of activity of esterase captured onto an immunoaffinity membrane

Youji Shimazaki, Takahiro Sakikawa, Ayaka Kimura

Journal:

Clinica Chimica Acta

Year:

2012

Language:

english

File:

PDF, 594 KB

english, 2012

22

Epitope analysis using membrane-immobilized avidin and protein A

Youji Shimazaki, Yoshinori Kohno, Izumi Fukui, Toshiharu Koyashiki

Journal:

Protein Expression and Purification

Year:

2012

Language:

english

File:

PDF, 626 KB

english, 2012

23

Enzyme separation and isozyme heterogeneity analysis using non-denaturing two-dimensional electrophoresis

Youji Shimazaki, Kenta Shimizu, Suzuna Masaoka

Journal:

Year:

2010

Language:

english

File:

PDF, 443 KB

english, 2010

24

Selection of an effective enzyme for digestion of non-denaturing proteins using microscale two-dimensional electrophoresis

Youji Shimazaki, Masayoshi Muro, Takashi Manabe

Journal:

Clinica Chimica Acta

Year:

2000

Language:

english

File:

PDF, 186 KB

english, 2000

25

Isolation of the third component of complement and its derivative with anaphylatoxin-like activity from the plasma of the newt Cynops pyrrhogaster

Youji Shimazaki, Kazutaka Maeyama, Tamotsu Fujii

Journal:

Developmental & Comparative Immunology

Year:

2001

Language:

english

File:

PDF, 184 KB

english, 2001

26

Removal of specific protein spots on the patterns of non-denaturing two-dimensional electrophoresis using protein A agarose and antibodies

Youji Shimazaki, Seiichi Ohara, Takashi Manabe

Journal:

Journal of Biochemical and Biophysical Methods

Year:

1998

Language:

english

File:

PDF, 157 KB

english, 1998

27

Protein spot recognition on the non-denaturing and denaturing two-dimensional electrophoresis patterns using in situ immunosubtraction via Protein A agarose and antibodies

Youji Shimazaki, Seiichi Ohara, Takashi Manabe

Journal:

Journal of Biochemical and Biophysical Methods

Year:

1999

Language:

english

File:

PDF, 264 KB

english, 1999

28

Simple quantification of Cu,Zn-superoxide dismutase activity after separation by non-denaturing isoelectric focusing

Youji Shimazaki, Hiromi Ohnishi, Satoshi Matsuura, Takashi Manabe

Journal:

Biochimica et Biophysica Acta (BBA) - General Subjects

Year:

2002

Language:

english

File:

PDF, 165 KB

english, 2002

29

Enzymolysis of high density lipoprotein with a combination of membrane-immobilized esterase and trypsin

Takahiro Sakikawa, Youji Shimazaki

Journal:

Journal of Pharmaceutical and Biomedical Analysis

Year:

2012

Language:

english

File:

PDF, 465 KB

english, 2012

30

Synthesis of 3-hydroxyretinal in the cytosol of the butterfly compound eye

Youji Shimazaki, Eisuke Eguchi

Journal:

Vision Research

Year:

1993

Language:

english

File:

PDF, 1.06 MB

english, 1993

31

344 Generation of anti-phosphorylated SNAP-25 antibody

Satoshi Iwasaki, Tei-ichi Nishiki, Mariko Sekiguchi, Youji Shimazaki, Masami Takahashi

Journal:

Neuroscience Research

Year:

1997

Language:

english

File:

PDF, 109 KB

english, 1997

32

A microfluidic device containing membrane-immobilized antibodies for successively capturing cytosolic enzymes

Shimazaki, Youji, Hashimoto, Ai

Journal:

Year:

2014

Language:

english

File:

PDF, 1018 KB

english, 2014

33

Analysis of trypsin inhibition activity in human plasma proteins after separation by non-denaturing two-dimensional electrophoresis

Shimazaki, Youji, Michhiro, Madoka

Journal:

Clinica Chimica Acta

Year:

2013

Language:

english

File:

PDF, 701 KB

english, 2013

34

Antigen digestion on the target plate of MALDI-TOF MS after isolation using an immunoaffinity membrane

Shimazaki, Youji, Nishimura, Yuri, Saito, Masaki

Journal:

Journal of Pharmaceutical and Biomedical Analysis

Year:

2013

Language:

english

File:

PDF, 876 KB

english, 2013

35

Micro-Scale Extraction and Analysis of Intact Carboxylesterase after Trapping on an Immunoaffinity Membrane Surface

Kimura, Ayaka, Shimazaki, Youji

Journal:

Applied Biochemistry and Biotechnology

Year:

2014

Language:

english

File:

PDF, 1.33 MB

english, 2014

36

Isolation and analysis of amyloid-β 1-42 monomer and oligomers in liquid droplets using an immunoaffinity membrane

Shimazaki, Youji, Hirose, Yu

Journal:

Journal of Chromatography B

Year:

2014

Language:

english

File:

PDF, 693 KB

english, 2014

37

Effect of Formoguanamine on Structure and Function of the Compound Eye of the Butterfly, Papilio xuthus

Miyako-Shimazaki, Yumiko, Shimazaki, Youji, Eguchi, Eisuke

Journal:

Zoological Science

Year:

1997

File:

PDF, 1.95 MB

1997

38

Analysis of enzyme activity regulation by non-denaturing electrophoresis and application of this regulation for enzyme reactor production

Shimazaki, Youji, Miki, Shizuka

Journal:

Journal of Enzyme Inhibition and Medicinal Chemistry

Year:

2013

Language:

english

File:

PDF, 717 KB

english, 2013

39

Combined Method of Immunoaffinity Membrane Within Tubes and MALDI-TOF MS for Capturing and Analyzing Amyloid Beta

Shimazaki, Youji, Takatsu, Yoko

Journal:

Applied Biochemistry and Biotechnology

Year:

2015

Language:

english

File:

PDF, 446 KB

english, 2015

40

Retaining activity of enzymes after capture and extraction within a single-drop of biological fluid using immunoaffinity membranes

Shimazaki, Youji, Sato, Yuki

Journal:

Journal of Chromatography B

Year:

2016

Language:

english

File:

PDF, 1.31 MB

english, 2016

41

Determination of Malic Acid Using a Malate Dehydrogenase Reactor After Purification and Immobilization in Non-Denaturing Conditions and Staining with Ponceau S

Shimazaki, Youji, Sakikawa, Takahiro

Journal:

Protein & Peptide Letters

Year:

2010

Language:

english

File:

PDF, 209 KB

english, 2010

42

Inhibition of amyloid β protein fibrillation via carboxypeptidase Y after protein trapping using immunoaffinity membranes

Saito, Masaki, Zako, Tamotsu, Takahashi, Ryoji, Shimazaki, Youji

Journal:

Chemistry Letters

Year:

2016

Language:

english

File:

PDF, 545 KB

english, 2016

43

Analytical Chemistry related to Biofunctional Research. Enzyme activity analysis of soluble proteins using non-denaturing two-dimensional electrophoresis.

SHIMAZAKI, Youji, HIRAKA, Yoko, OHNISHI, Hiromi, MANABE, Takashi

Journal:

Year:

2002

File:

PDF, 2.64 MB

2002

44

Hydrolytic Activity of Esterase-Antibody Complexes Retained Within Gel Capsules After Complex Isolation

Shimazaki, Youji, Miyatsuka, Rino

Journal:

Applied Biochemistry and Biotechnology

Year:

2017

Language:

english

File:

PDF, 1.13 MB

english, 2017

45

Enzyme Inhibitory Activity of Ovomucoid Extracted Using a Carboxypeptidase Y-Immobilized Membrane

SHIMAZAKI, Youji, TANAKA, Kosuke, SAKATA, Keisuke

Journal:

Year:

2018

Language:

english

File:

PDF, 1.60 MB

english, 2018

46

Antibacterial activity of lysozyme-binding proteins from chicken egg white

Shimazaki, Youji, Takahashi, Ayumi

Journal:

Journal of Microbiological Methods

Year:

2018

Language:

english

File:

PDF, 903 KB

english, 2018

47

Removal of Copper in Microdroplets by Ovomucoid Hydrolysates Bound to Reverse-Phase Chromatography Media Within Pipette Tips

Shimazaki, Youji, Inoue, Suzuka

Journal:

Applied Biochemistry and Biotechnology

Year:

2019

Language:

english

File:

PDF, 963 KB

english, 2019

48

Analysis of the degradation of amyloid beta fibrils after separation via the combination of non‐denaturing agarose electrophoresis and Congo red dye staining

Kawano, Risa, Nakanishi, Ayaka, Zako, Tamotsu, Shimazaki, Youji

Journal:

Separation Science Plus

Year:

2019

Language:

english

File:

PDF, 527 KB

english, 2019

49

Electrophoretic injection and reaction of dye-bound enzymes to protein and bacteria within gel

Shimazaki, Youji, Inoue, Aoshi, Ikeuchi, Haruka

Journal:

Journal of Microbiological Methods

Year:

2020

File:

PDF, 528 KB

2020

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